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Image Search Results
Journal: eLife
Article Title: Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte function
doi: 10.7554/eLife.84168
Figure Lengend Snippet:
Article Snippet: Antibody ,
Techniques: Recombinant, Plasmid Preparation, Sequencing, TaqMan Assay, DNA Extraction, Isolation, Sample Prep, Picogreen Assay
Journal: Aging (Albany NY)
Article Title: Testosterone enhances mitochondrial complex V function in the substantia nigra of aged male rats
doi: 10.18632/aging.103265
Figure Lengend Snippet: Effects of TP supplementation on complex V subunit expression in the substantia nigra of aged male rats. ( A – C ) The mRNA levels of ATP6 , ATP8 and ATP5C1 were calculated using the 2 -ΔΔCt method. GAPDH was used as an internal control. ( D – F ) Representative Western blots of ATP6, ATP8 and ATP5C1 protein levels. ( G – I ) ATP6, ATP8 and ATP5C1 protein levels were quantified by comparing the band density of each protein to that of β-actin (endogenous control). Data are expressed as the mean ± S.D. (n=6 rats/group). * P <0.05, ** P <0.01.
Article Snippet: The membrane was rinsed three times with TBST and then incubated overnight with a rabbit anti-ATP6 polyclonal antibody (1:500, A8193, ABclonal), rabbit anti-ATP8 polyclonal antibody (1:200, GTX55993, GeneTex),
Techniques: Expressing, Western Blot
Journal: Aging (Albany NY)
Article Title: Testosterone enhances mitochondrial complex V function in the substantia nigra of aged male rats
doi: 10.18632/aging.103265
Figure Lengend Snippet: Effects of TP supplementation on complex V subunit mRNA levels in the substantia nigra of aged male rats.
Article Snippet: The membrane was rinsed three times with TBST and then incubated overnight with a rabbit anti-ATP6 polyclonal antibody (1:500, A8193, ABclonal), rabbit anti-ATP8 polyclonal antibody (1:200, GTX55993, GeneTex),
Techniques:
Journal: Aging (Albany NY)
Article Title: Testosterone enhances mitochondrial complex V function in the substantia nigra of aged male rats
doi: 10.18632/aging.103265
Figure Lengend Snippet: Effects of gonadectomy and TP replacement on mitochondrial complex V subunit expression in the substantia nigra of adult male rats. ( A – E ) The mRNA levels of ATP6 , ATP8 , ATP5C1 , ATP5I and ATP5L were calculated using the 2 -ΔΔCt method. GAPDH was used as an internal control. ( F – J ) Representative Western blots of ATP6, ATP8, ATP5C1, ATP5I and ATP5L protein levels. ( K – O ) ATP6, ATP8, ATP5C1, ATP5I and ATP5L protein levels were quantified by comparing the band density of each protein to that of β-actin (endogenous control). Data are expressed as the mean ± S.D. (n=6 rats/group). * P <0.05, ** P <0.01.
Article Snippet: The membrane was rinsed three times with TBST and then incubated overnight with a rabbit anti-ATP6 polyclonal antibody (1:500, A8193, ABclonal), rabbit anti-ATP8 polyclonal antibody (1:200, GTX55993, GeneTex),
Techniques: Expressing, Western Blot
Journal: Aging (Albany NY)
Article Title: Testosterone enhances mitochondrial complex V function in the substantia nigra of aged male rats
doi: 10.18632/aging.103265
Figure Lengend Snippet: Effects of gonadectomy and TP replacement on complex V subunit mRNA levels in the substantia nigra of adult male rats.
Article Snippet: The membrane was rinsed three times with TBST and then incubated overnight with a rabbit anti-ATP6 polyclonal antibody (1:500, A8193, ABclonal), rabbit anti-ATP8 polyclonal antibody (1:200, GTX55993, GeneTex),
Techniques: